Calcium Colorimetric Assay Kit

Description: Protein phosphorylation is one of the most important post-translational_x000D_modification processes. Phosphorylation is reversibly regulated by Protein Kinases (PKs) and_x000D_Protein Phosphatases (PTPs). PTP1B (PTPN1) is known to catalyze dephosphorylation of_x000D_insulin receptor kinases and plays a critical role in insulin signaling. The PTP1B Colorimetric_x000D_Assay Kit is designed to measure PTP1B activity for screening and profiling applications, in a_x000D_homogeneous assay with no time-consuming washing steps. The PTP1B Colorimetric Assay Kit is optimized for both_x000D_continuous and end-point assay formats.

Description: The PARP1 Colorimetric Activity Assay Kit is designed to measure PARP1_x000D_activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependent_x000D_addition of poly(ADP-ribose) to histones. The key to the PARP1 Colorimetric Activity_x000D_Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1_x000D_reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated_x000D_substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate_x000D_is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to_x000D_produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.

Description: The PARP2 Colorimetric Activity Assay Kit is designed to measure PARP2_x000D_activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent_x000D_addition of poly(ADP-ribose) to histones. The key to the PARP2 Colorimetric Activity_x000D_Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP2_x000D_reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP2 biotinylated_x000D_substrate is incubated with an assay buffer that contains the PARP2 enzyme. Finally, the plate_x000D_is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to_x000D_produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.

Description: This product includes 1 ml of 10x Assay buffer, 0.2 ml of 10 x Reagent A, 0.2 ml of 10 x Reagent B and 0.5 ml of 10x Reagent C.