Chemiluminescent/Fluo substrat for

Description: Cyclic AMP (cAMP) is an important regulatory molecule in the GPCR signaling cascade. Our cAMP ELISA Kits provide a highly sensitive method to measure low levels of cyclic AMP in a variety of sample types.

Description: Cyclic AMP (cAMP) is an important regulatory molecule in the GPCR signaling cascade. Our cAMP ELISA Kits provide a highly sensitive method to measure low levels of cyclic AMP in a variety of sample types.

Description: Cyclic GMP (cGMP) is an important regulatory molecule in the GPCR signaling cascade. Our cGMP ELISA Kits provide a highly sensitive method to measure low levels of cyclic GMP in a variety of sample types.

Description: Cyclic GMP (cGMP) is an important regulatory molecule in the GPCR signaling cascade. Our cGMP ELISA Kits provide a highly sensitive method to measure low levels of cyclic GMP in a variety of sample types.

Description: The PARP12 Chemiluminescent Assay Kit is designed to measure PARP12 activity for screening and profiling applications. PARP12 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the PARP12 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP12 reactions. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP12 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The FTO Chemiluminescent Assay Kit is designed to measure FTO activity for screening and profiling applications. The key to the FTO Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to the activity. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, FTO enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The NSD3 Chemiluminescent Assay Kit is designed to measure NSD3 activity for screening and profiling applications. The key to the NSD3 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The NSD2 Chemiluminescent Assay Kit is designed to measure NSD2 activity for screening and profiling applications. The key to the NSD2 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The METTL3 Chemiluminescent Assay Kit is designed to measure METTL3 activity for screening and profiling applications. The METTL3 Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated adenosine, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified METTL3 enzyme for 96 enzyme reactions. The key to the METTL3 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes N6methylated adenosine. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PRDM9 Chemiluminescent Assay Kit is designed to measure PRDM9 activity for screening and profiling applications, using purified PRDM9. The PRDM9 Chemiluminescent Assay Kit comes in a convenient format, with 8-well strips pre-coated with histone H3 peptide substrate, an antibody against methylated lysine on Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and enough purified PRDM9 enzyme for 100 enzyme reactions. The key to the PRDM9 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The ALKBH5 Chemiluminescent Assay Kit is designed to measure ALKBH5 activity for screening and profiling applications. The key to the ALKBH5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to ALKBH5 demethylase activity. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, ALKBH5 enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The p300 Chemiluminescent Assay Kit is an enzyme-linked immunosorbent assay (ELISA) designed to screen for inhibitors of p300 or to measure histone acetyltransferase (HAT) activity for screening and profiling applications. Histone acetyltransferase p300 (also known as KAT3B) is involved in various cellular events, and its dysfunction is linked to a number of human diseases including Parkinson's, Huntington's, and Alzheimer's diseases. The P300 Chemiluminescent Assay Kit is based on the p300 enzyme transferring an acetyl group from an acetyl donor (acetyl CoA) to a histone substrate. The acetylated histone is recognized by a highly specific primary antibody, followed by an HRP-labeled secondary antibody. The chemiluminescence produced by HRP can be measured using a chemiluminescence reader._x000D_

Description: The PARP7 Chemiluminescent Activity Assay kit is designed to measure PARP7 activity for screening and profiling applications. The key to the PARP7 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP7 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PARP7 Chemiluminescent Activity Assay Kit is designed to measure PARP7 activity for screening and profiling applications. PARP7 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones. With this kit, only three simple steps are required for PARP7 reactions. First, histone proteins are coated on a 384-well plate. Next, the PARP7 biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PARP1 Chemiluminescent Activity Assay kit is designed to measure PARP1 activity for screening and profiling applications. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with the assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PARP2 Chemiluminescent Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP2 enzyme and an activated DNA template in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.*NOTE: As of March 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

Description: The PARP2 Chemiluminescent Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP2 enzyme and an activated DNA template in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.*NOTE: As of March 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

Description: The PARP3 Chemiluminescent Activity Assay kit is designed to measure PARP3 activity for screening and profiling applications. The key to the PARP3 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP3 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with the assay buffer that contains the PARP3 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PARP6 Chemiluminescent Activity Assay kit is designed to measure PARP6 activity for screening and profiling applications. The key to the PARP6 chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP6 reactions. First, histone proteins are coated on a microplate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP6 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PARP10 Chemiluminescent Assay Kit is designed to measure PARP10 activity for screening and profiling applications. The key to the PARP10 Chemiluminescent Assay Kit is the biotinylated substrate. With this kit, only three simple steps are required for PARP10 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP10 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader

Description: The PARP11 Chemiluminescent Activity Assay kit is designed to measure PARP11 activity for screening and profiling applications. The key to the PARP11 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP11 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP11 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PARP15 Chemiluminescent Assay Kit is designed to measure PARP15 activity for screening and profiling applications. The key to the PARP15 Chemiluminescent Assay Kit is the biotinylated substrate. With this kit, only three simple steps are required for PARP15 reactions. First, histone proteins are coated onto the wells of the strip-plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP15 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PARP14 Chemiluminescent Activity Assay Kit is designed to measure PARP14 activity for screening and profiling applications. The key to the PARP14 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP14 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated substrate is incubated with an assay buffer that contains the PARP14 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader

Description: The GCN5 Chemiluminescent Assay Kit is an enzyme-linked immunosorbent assay (ELISA) designed to screen for inhibitors of GCN5. The GCN5 Chemiluminescent Assay Kit is based on the GCN5 enzyme transferring an acetyl group from an acetyl donor (acetyl CoA) to a histone substrate. The acetylated histone is recognized by a highly specific primary antibody, followed by an HRP-labeled secondary antibody. The chemiluminescence produced by HRP can be measured using a chemiluminescence reader. _x000D_

Description: The UTX Chemiluminescent Assay Kit is designed to measure UTX activity for screening and profiling applications. UTX, also known as KDM6A, is a JmjC-domain protein that exhibits demethylation activity toward di- and trimethyl-lysine 27 on histone H3 (H3K27me2/3).The key to the UTX Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, UTX enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The TET1 Chemiluminescent Assay Kit is designed to measure TET1 activity for screening and profiling applications. TET1 belongs to the Ten Eleven Translocation (TET) family proteins that catalyze 5-methylcytosine oxidation and generate 5-methylcytosine derivatives, including 5-hydroxymethylcytosine. TET1 may repress gene transcription in a catalytic activity-independent manner_x000D__x000D_ The key to the TET1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes hydroxymethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of TET1 activity. First, the TET1 enzyme is incubated with the methylated substrate for two hours. Then the primary antibody is added. Next, the plate is treated with an HRP-labeled secondary antibody. Finally, the HRP substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. _x000D_

Description: The TET2 Chemiluminescent Assay Kit is designed to measure TET2 activity for screening and profiling applications. TET2 belongs to the Ten Eleven Translocation (TET) family proteins that catalyze 5-methylcytosine oxidation and generate 5-methylcytosine derivatives, including 5-hydroxymethylcytosine. TET2 is one of the most frequently mutated genes in myeloid malignancies. _x000D__x000D__x000D__x000D_ The key to the TET2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes hydroxymethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of TET2 activity. First, TET2 enzyme is incubated with the methylated substrate for two hours. Then the primary antibody is added. Next, the plate is treated with an HRP-labeled secondary antibody. Finally, the HRP substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1_x000D_activity for screening and profiling applications. The key to the SetDB1 Direct Activity Assay Kit is a highly specific antibody that_x000D_recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are_x000D_required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample_x000D_containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally,_x000D_the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP_x000D_substrate to produce chemiluminescence that can then be measured using a chemiluminescence_x000D_reader.

Description: The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The advantage of the SetDB1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required. First, S-adenosylmethionine is incubated with a sample containing the assay buffer and the methyltransferase enzyme. Next, the primary antibody is added. Finally, the wells are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The advantage of the SetDB1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required. First, S-adenosylmethionine is incubated with a sample containing the assay buffer and the methyltransferase enzyme. Next, the primary antibody is added. Finally, the wells are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The G9a Direct Activity Chemiluminescent Assay Kit is designed to measure G9a_x000D_activity for screening and profiling applications. The key to the G9a Direct Activity Assay_x000D_Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With_x000D_this kit, only three simple steps are required for methyltransferase detection. First,_x000D_S-adenosylmethionine is incubated with a sample containing assay buffer_x000D_and methyltransferase enzyme for one hour. Next, primary antibody is added._x000D_Finally, the strips are treated with an HRP-labeled secondary antibody followed by_x000D_addition of the HRP substrate to produce chemiluminescence that can then be measured_x000D_using a chemiluminescence reader.