Hela-GFP stable cells Puromycin

Description: GFP expression stable cell line in ZR-75-1 cells with Puromycin resistance

Description: Transformed from Hela cells by lentiviral system with sequence verified firefly luciferase gene II and GFP. The Luciferase and GFP was co-expressed under CMV promoter as seperated protein(not as fusion). The Puromycin marker was expressed under Rsv promoter.

Description: Transformed from Hela cells by lentiviral system with sequence verified firefly luciferase gene. Luciferase was expressed under CMV promoter, A Puromycin antibiotic marker was expressed under Rsv promoter. The cell constitutively express Luciferase enzyme.

Description: Luciferase (firefry) and GFP co-expression stable cell line in A549 human cancer cell line with Puromycin marker.

Description: Transformed from Hela cells by lentiviral system with sequence verified firefly luciferase gene II and RFP. The Luciferase and RFP was co-expressed under CMV promoter as seperated protein(not as fusion). The Puromycin marker was expressed under Rsv promoter.

Description: Luciferase (firefry) expression stable cell line in A549 human cancer cell line with Puromycin marker.

Description: N/A

Description: Luciferase (firefly) and CRE recombinase co-expression stable cell line with Puromycin marker.

Description: tetracycline repressor expression Hela stable cell line with Puromycin marker

Description: GFP expression stable cell line in Hela cells with Blasticidin marker.

Description: rtTA (reverse tetracycline Transcriptional Activator) expression Hela stable cell line with GFP-Blasticidin fusion dual marker

Description: rtTA (reverse tetracycline Transcriptional Activator) expression Hela stable cell line with GFP-Puromycin fusion dual marker

Description: Enhanced green fluorescent protein (eGFP) is a modified (F64L and S65T mutations) version of the native GFP protein isolated from jellyfish (Aequorea victoria), with increased fluorescence and more efficient folding. The Enhanced GFP Lentivirus are replication-incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. These viruses constitutively express eGFP under a CMV promoter. eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, and extinction coefficient of 55,000 M^-1cm^-1.

Description: Extracted from Streptomyces alboniger bacteria, Puromycin is an aminonucleoside antibiotic that inhibits protein synthesis by interfering with the translation process. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. The antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells. Fungi and Gram negative bacteria are resistant to Puromycin due to its low permeability. But in some particular conditions puromycin can be used for E. coli. For more than 30 years, puromycin has been widely used as a basic tool for studying protein synthesis. Now, puromycin hydrochloride is particularly useful for the selection of cell types harbouring plasmids carrying puromycin resistance genes. ;RESISTANCE TO PUROMYCIN;

The pac gene encoding a Puromycin N-acetyl-transferase (PAC) has been isolated from a Streptomyces productin strain^1,2. It is located in a region of the pur cluster linked to the other genes determining the puromycin biosynthetic pathway. The expression of pac gene confers puromycin resistance to transfected mammalian cells³ expressing it. Under some conditions puromycin could also be used for selection of E. coli strains transformed with plasmids carrying the pac gene.

CONDITIONS OF SELECTION

Puromycin is poorly active on E. coli but is particularly useful in experiments conducted with mammalian cells. It can be used as an alternative to the Neomycin system for transfection experiments.

Mammalian cells

The working concentrations of puromycin for mammalian cell lines range from 1 to 10µg/mL. In a starting experiment we recommend to determine optimal concentrations of antibiotic required to kill your host cell line. Puromycin quickly kills eukaryotic cells that do not contain the pac gene. Dying cells detach form the plates allowing easy and early identification of transformant clones.

References:

1) VARA J., et al. (1985). Biochemistry. 24: 8074-8081

2) LACALLE R.A., et al. (1989). Gene. 79: 375-380

3) DE LA LUNA S and J. ORTIN. (1992). Methods In Enzymology. 216: 376-385

Description: 53-79-2

Description: 53-79-2

Description: Puromycin

Description: Puromycin

Description: Puromycin

Description: Puromycin

Description: Puromycin

Description: Transformed from HEK293 cells, expressing firefly luciferase gene II. Luciferase and GFP was individually co-expressed under CMV promoter, The Puromycin marker was expressed under Rsv promoter. The cell constitutively co-expresses both Luciferase and GFP.

Description: RFP expression stable cell line in Hela cells with Puromycin marker.

Description: Transformed from huamn lung cancer cell line, A549 by lentiviral system with firefly luciferase gene II and GFP. The Luciferase and GFP was co-expressed under CMV promoter as seperated protein(not as fusion). The Puromycin marker was expressed under Rsv promoter. The cell constitutively co-expresses both firefly Luciferase and GFP. Each cell demonstrates strong GFP fluorescent signal and luciferase assay signal(Note: this is pooled stable ell population, not originated from a single cell colony. Thus you will observe the uneven GFP signal intensity amount cells.

Description: Puromycin antibiotic selection solution in PBS

Description: The STAT3 Reporter (Luc)-HEK293 cell line is designed for monitoring STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines and growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

Description: Concentrated lentivirus co-express (GFP and Puromycin) under an Enhanced EF1a promoter, provided in PBS solution premixed with polybrene.

Description: Luciferase (firefry) and GFP co-expression stable cell line in Hela cells with Neomycin marker.

Description: Modified Nucleotides

Description: Modified Nucleotides

Description: Modified Nucleotides

Description: DNA Damage/DNA Repair|DNA Synthesis

Description: DNA Damage/DNA Repair|DNA Synthesis

Description: DNA Damage/DNA Repair|DNA Synthesis

Description: Biotechnology

Description: Biotechnology

Description: Biotechnology

Description: Others|Others

Description: Others|Others

Description: Others|Others

Description: Puromycin dihydrochloride

Description: Puromycin aminonucleoside (NSC 3056) is the aminonucleoside portion of the antibiotic puromycin, and used in nephrosis animal models[1]. Puromycin aminonucleoside induces apoptosis[2]. Puromycin aminonucleoside is a reversible inhibitor of dipeptidyl peptidase II and cytosol alanyl aminopeptidase[3]. Puromycin aminonucleoside induces secretion of cell migrasome[4].

Description: 58-58-2

Description: 58-60-6

Description: 58-60-6

Description: 58-60-6

Description: 58-58-2

Description: 58-58-2

Description: 58-58-2

Description: 58-58-2

Description: Puromycin aminonucleoside

Description: Puromycin aminonucleoside

Description: Puromycin aminonucleoside

Description: Puromycin aminonucleoside

Description: Puromycin aminonucleoside

Description: Puromycin dihydrochloride

Description: Puromycin dihydrochloride

Description: Puromycin dihydrochloride

Description: Puromycin dihydrochloride

Description: Puromycin dihydrochloride

Description: Puromycin dihydrochloride

Description: Luciferase (firefry) and GFP co-expression stable cell line in Hela cells with Blasticidin marker.

Description: Transformed from huamn lung cancer cell line, A549 by lentiviral system with firefly luciferase gene. The Luciferase was under modified CMV promoter. The Puromycin marker was expressed under Rsv promoter. The cell line demonstrates high level of bioluminescence signal via firefly luciferase assay.

Description: Transformed from human breast cancer cell line, MCF7 adhesive cell using lentivirus expressing firefly luciferase gene. The Luciferase was expressed under the enhanced EF1a promoter. The Puromycin marker was expressed under Rsv promoter. The cell line demonstrates high level of bioluminescence signal via firefly luciferase assay.

Description: rtTA (reverse tetracycline Transcriptional Activator) expression Hela stable cell line with RFP-Blasticidin fusion dual marker

Description: Unconjugated

Description: Unconjugated

Description: Unconjugated

Description: Unconjugated

Description: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Description: Transformed from human human pancreatic cancer cell line, CFPAC-1 adhesive epithelial cell, using lentivirus expressing firefly luciferase 3 gene. The Luciferase was expressed under the enhanced EF1a promoter. The Puromycin marker was expressed under Rsv promoter. The cell line demonstrates high level of bioluminescence signal via firefly luciferase assay.

Description: Transformed from human Jurkat T lymphocyte suspension cells using lentivirus expressing firefly luciferase gene. The Luciferase was expressed under the enhanced EF1a promoter. The Puromycin marker was expressed under Rsv promoter. The cell line demonstrates high level of bioluminescence signal via firefly luciferase assay.

Description: Transformed from HEK293 cells by lentiviral system with sequence verified firefly luciferase gene II and CRE recombinase. Luciferase and CRE was co-expressed under CMV promoter as seperated protein(not as fusion). The Puromycin marker was expressed under Rsv promoter. The cell constitutively co-expresses both firefly Luciferase and CRE.

Description: Transformed from human non-small cell lung carcinoma cell line, NCI-H1299 adhesive cell, using lentivirus expressing firefly luciferase 3 gene. The Luciferase was expressed under the enhanced EF1a promoter. The Puromycin marker was expressed under Rsv promoter. The cell line demonstrates high level of bioluminescence signal via firefly luciferase assay.

Description: Transformed from human breast epithelial cancer cell line, ZR-75-1 adhesive cell using lentivirus expressing firefly luciferase gene. The Luciferase was expressed under the enhanced EF1a promoter. The Puromycin marker was expressed under Rsv promoter. The cell line demonstrates high level of bioluminescence signal via firefly luciferase assay.

Description: Transformed from HEK293 cells, expressing the firefly luciferase gene. Luciferase was expressed under CMV promoter, A Puromycin antibiotic marker was expressed under Rsv promoter. The cell constitutively express Luciferase enzyme.

Description: Transformed from HEK293 cells by lentiviral system with sequence verified Renilla Luciferase. Renilla luciferase was expressed under CMV promoter, A Puromycin antibiotic marker was expressed under Rsv promoter. The cell constitutively express Renilla luciferase enzyme.

Description: Part B

Description: Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene._x000D_
Cas9 Lentivirus can be used to generate Cas9 expressing cells in almost any mammalian cell line. Cells stably expressing Cas9 can then be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines._x000D_
The Cas9 Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a Cas9 gene driven by an EF1a promoter, along with a Puromycin selection marker._x000D_

Description: Transformed from HEK293 cells, expressing the firefly luciferase gene. Luciferase and RFP was co-expressed under CMV promoter as individual protein(not as fusion). The Puromycin marker was expressed under Rsv promoter. The cell constitutively express both Luciferase and RFP.

Description: Luciferase (firefry) expression stable cell line in Hela cells with Blasticidin marker.

Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Puromycin marker under Rsv promoter.

Description: Puromycin-bis(PEG2-amide)-Biotin is used for single-cell translatome sequencing[1].

Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Puromycin marker under Rsv promoter.

Description: The Renilla Luciferase (Rluc) Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles constitutively express Renilla luciferase under a CMV promoter.

Description: The Firefly Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. This virus constitutively express firefly luciferase under a CMV promoter.

Description: Human TRPC3 ion-channel stable cell line with RFP-Blasticidin dual marker.

Description: Human KCNN4 ion-channel stable cell line with RFP-Blasticidin dual marker.

Description: Human TRPV1 ion-channel stable cell line with RFP-Blasticidin dual marker.

Description: Human ATP2B2 ion-channel stable cell line with RFP-Blasticidin dual marker.

Description: Media Catalog; Cell Culture Reagents

Description: Luciferase (firefry) and RFP co-expression stable cell line in Hela cells with Neomycin marker.

Description: Red-Luciferease expression lentivirus under EF1a promoter, containing Puromycin selection.

Description: Luciferase (firefry) and RFP co-expression stable cell line in Hela cells with Blasticidin marker.

Description: Recombinant Human Puromycin Sensitive Aminopeptidase expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells