Interrelations between gene expression and DNA methylation patterns in bovine mammary gland

21 września, 2020 Dominika Zostaw komentarz

Quick Communication: Locus-specific interrelations between gene expression and DNA methylation patterns in bovine mammary gland contaminated by coagulase-positive and coagulase-negative staphylococci

Pathogens are in a position to alter the cell cycle program and immune response of the host by altering the transcription and epigenetics of genes liable for cell cycle management and irritation. On this regard, we evaluated interrelations between DNA methylation and expression of autophagy, apoptosis, and lipid metabolism-related genes in a pattern set of mammary gland secretory tissue sections derived from bovine mammary glands contaminated with coagulase-negative and coagulase-positive staphylococci.

We assessed relative transcript abundance and DNA bisulfite sequencing in loci of the ATG5, IGF1R, TERT, and DGAT1 genes. Lack of DNA methylation in ATG5 and DGAT1 loci is perhaps related to upkeep of ATG5 and DGAT1 expression whatever the well being standing of bovine mammary gland. Full methylation of intragenic CpG areas within the IGF1R locus was apparently not associated to the presence of its transcript within the investigated udder parenchyma samples.

Detected hypermethylation of the TERT upstream ingredient was related to a small quantity of TERT mRNA in bovine mammary gland, whatever the presence, or absence, of the pathogen. A big lower in TERT gene expression in tissue sections of mammary gland freed from micro organism and in these contaminated with coagulase-positive staphylococci was noticed in parenchyma samples contaminated with coagulase-negative staphylococci.

Two attainable explanations are the direct involvement of the TERT gene within the etiology of bovine mastitis or the rise of TERT mRNA because of activation of the MAPK signaling pathway in response to launch of exotoxins by coagulase-negative micro organism within the bovine mammary gland.

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Quick communication: Locus-specific interrelations between gene expression and DNA methylation patterns in bovine mammary gland contaminated by coagulase-positive and coagulase-negative staphylococci

We assessed relative transcript abundance and DNA bisulfite sequencing in loci of the ATG5, IGF1R, TERT, and DGAT1 genes. Lack of DNA methylation in ATG5 and DGAT1 loci is perhaps related to upkeep of ATG5 and DGAT1 expression whatever the well being standing of bovine mammary gland. Full methylation of intragenic CpG areas within the IGF1R locus was apparently not associated to the presence of its transcript within the investigated udder parenchyma samples. Detected hypermethylation of the TERT upstream ingredient was related to a small quantity of TERT mRNA in bovine mammary gland, whatever the presence, or absence, of the pathogen.

A big lower in TERT gene expression in tissue sections of mammary gland freed from micro organism and in these contaminated with coagulase-positive staphylococci was noticed in parenchyma samples contaminated with coagulase-negative staphylococci. Two attainable explanations are the direct involvement of the TERT gene within the etiology of bovine mastitis or the rise of TERT mRNA because of activation of the MAPK signaling pathway in response to launch of exotoxins by coagulase-negative micro organism within the bovine mammary gland.

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Viral DNA / RNA Kit (with Magnetic Stand)
abx098871-50rxns	Abbexa	50 rxns	477.6 EUR

Viral DNA/RNA Kit (with Magnetic Stand)
abx298029-50rxns	Abbexa	50 rxns	477.6 EUR

Bulk Beads
D1131-01	Benchmark Scientific	1 PC	285.76 EUR

Bulk Beads
D1131-05	Benchmark Scientific	1 PC	285.76 EUR

Bulk Beads
D1131-10	Benchmark Scientific	1 PC	492.47 EUR

Bulk Beads
D1132-01TP	Benchmark Scientific	1 PC	492.47 EUR

Bulk Beads
D1132-05TP	Benchmark Scientific	1 PC	492.47 EUR

Bulk Beads
D1132-10TP	Benchmark Scientific	1 PC	492.47 EUR

Bulk Beads
D1132-15TP	Benchmark Scientific	1 PC	492.47 EUR

Bulk Beads
D1132-30TP	Benchmark Scientific	1 PC	492.47 EUR

Bulk Beads
D1132-60	Benchmark Scientific	1 PC	289.93 EUR

Bulk Beads
D1133-28	Benchmark Scientific	1 PC	112.45 EUR

Exorose Beads
EXOB-100	ABTBeads	100 ml	127.2 EUR
Exorose Beads
EXOB-25	ABTBeads	25 ml	69.6 EUR
Exorose Beads
EXOB-250	ABTBeads	250 ml	225.6 EUR
Latex Beads
abx291003-1L	Abbexa	1 L	2798.4 EUR

RNA Beads
20-abx298002	Abbexa	260.40 EUR 477.60 EUR 1663.20 EUR	1 ml 5 ml 60 ml

Saline & Beads
MED1064	Scientific Laboratory Supplies	PK50	91.2 EUR
V5 agarose beads
AE029-100ul	Abclonal	100 ul	Ask for price
V5 agarose beads
AE029-200ul	Abclonal	200 ul	273.6 EUR
V5 agarose beads
AE029-20ul	Abclonal	20 ul	Ask for price
V5 agarose beads
AE029-50ul	Abclonal	50 ul	Ask for price
Refill Glass Beads
B1201-BEAD	Benchmark Scientific	1 PC	141.34 EUR

Glass Beads 0.1mm
GB01	Next Advance	1pack	129.6 EUR
Description: Glass beads. 0.1mm. 1 lb. Non-sterile.
Glass Beads 0.5mm
GB05	Next Advance	1pack	123.6 EUR
Description: Glass beads. 0.5mm. 1 lb. Non-sterile.
Glass Beads 1.0mm
GB10	Next Advance	1pack	123.6 EUR
Description: Glass beads. 1.0mm. 1 lb. Non-sterile.
Sodium hydroxide, Beads
SB6789	Bio Basic	500g	73.21 EUR

RanBP1 Agarose Beads
STA-421	Cell Biolabs	400 µg	686.4 EUR
Description: RanBP1 Agarose Beads selectively pull down the active form of Ran. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.
Protect beads green
SWA1151	Scientific Laboratory Supplies	PK80	103.74 EUR
Protect Beads White
SWA1155	Scientific Laboratory Supplies	PK80	103.74 EUR
Protect Beads Blue
SWA1158	Scientific Laboratory Supplies	PK80	103.74 EUR
Streptavidin-Sepharose Beads
6565-10	Biovision	each	836.4 EUR
Streptavidin-Sepharose Beads
6565-100	Biovision	each	Ask for price
Streptavidin-Sepharose Beads
6565-2	Biovision	each	274.8 EUR
Streptavidin-Sepharose Beads
6565-5	Biovision	each	483.6 EUR
EZEnrich? Polyubiquitin Beads
6568-300	Biovision	each	444 EUR
Thrombin Sepharose Beads
7925-1	Biovision	each	261.6 EUR
Thrombin Sepharose Beads
7925-25	Biovision	each	2114.4 EUR
Thrombin Sepharose Beads
7925-5	Biovision	each	836.4 EUR
Plasmin Sepharose Beads
7926-1	Biovision	each	261.6 EUR
Plasmin Sepharose Beads
7926-25	Biovision	each	2114.4 EUR
Plasmin Sepharose Beads
7926-5	Biovision	each	836.4 EUR
Urokinase Sepharose Beads
7927-1	Biovision	each	261.6 EUR
Urokinase Sepharose Beads
7927-25	Biovision	each	2114.4 EUR
Urokinase Sepharose Beads
7927-5	Biovision	each	836.4 EUR
Immobilized Catalase Beads
7931-1	Biovision	each	183.6 EUR
Immobilized Catalase Beads
7931-10	Biovision	each	705.6 EUR
Calmodulin-Sepharose Beads
7934-1	Biovision	each	183.6 EUR
Calmodulin-Sepharose Beads
7934-10	Biovision	each	574.8 EUR
Calmodulin-Sepharose Beads
7934-5	Biovision	each	405.6 EUR
VAHTS mRNA Capture Beads
N401-01	Vazyme	24 rxn	171.6 EUR
VAHTS mRNA Capture Beads
N401-02	Vazyme	96 rxn	306 EUR
VAHTS RNA Clean Beads
N412-01	Vazyme	5 ml	286.8 EUR
VAHTS RNA Clean Beads
N412-02	Vazyme	40 ml	495.6 EUR
VAHTS RNA Clean Beads
N412-03	Vazyme	450 ml	4860 EUR
Stainless Steel Beads 0.2mm
SSB02	Next Advance	1pack	229.2 EUR
Description: Stainless steel beads. 0.2mm. 1 lb. Non-sterile.
Stainless Steel Beads 0.5mm
SSB05	Next Advance	1pack	229.2 EUR
Description: Stainless steel beads. 0.5mm. 1 lb. Non-sterile.
Stainless Steel Beads 1.6mm
SSB16	Next Advance	1pack	304.8 EUR
Description: Stainless steel beads. 1.6mm. 1 lb. Non-sterile.
Stainless Steel Beads 2.3mm
SSB23	Next Advance	1pack	260.4 EUR
Description: Stainless steel beads. 2.3mm. 1 lb. Non-sterile.
Stainless Steel Beads 3.2mm
SSB32	Next Advance	1pack	220.8 EUR
Description: Stainless steel beads. 3.2mm. 1 lb. Non-sterile.
Stainless Steel Beads 4.8mm
SSB48	Next Advance	1pack	220.8 EUR
Description: Stainless steel beads. 4.8mm. 1 lb. Non-sterile.
Stainless Steel Beads 6.0mm
SSB60	Next Advance	1pack	223.2 EUR
Description: Stainless steel beads. 6.0mm. 1 lb. Non-sterile.
PAK1 PBD Agarose Beads
STA-411	Cell Biolabs	400 ?g	686.4 EUR
Description: PAK1 PBD Agarose Beads selectively pull down the active form of Rac and Cdc42. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.
Rhotekin RBD Agarose Beads
STA-412	Cell Biolabs	400 ?g	686.4 EUR
Description: Rhotekin RBD Agarose Beads selectively pull down the active form of Rho. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.
RalGDS RBD Agarose Beads
STA-418	Cell Biolabs	400 µg	686.4 EUR
Description: RalGDS RBD Agarose Beads selectively pull down the active form of Rap. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.
GGA3 PBD Agarose Beads
STA-419	Cell Biolabs	400 µg	686.4 EUR
Description: GGA3 PBD Agarose Beads selectively pull down the active form of Arf. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.
RalBP1 PBD Agarose Beads
STA-420	Cell Biolabs	400 µg	686.4 EUR
Description: RalBP1 RBD Agarose Beads selectively pull down the active form of Ral. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.
Zirconium Oxide Beads 0.15mm
ZrOB015	Next Advance	1pack	189.6 EUR
Description: Fused Zirconium Oxide beads . 0.15mm. 1 lb. Non-sterile.
Zirconium Oxide Beads 0.5mm
ZrOB05	Next Advance	1pack	189.6 EUR
Description: Zirconium Oxide beads . 0.5mm. 1 lb. Non-sterile.
Zirconium Oxide Beads 1.0mm
ZrOB10	Next Advance	1pack	189.6 EUR
Description: Zirconium Oxide beads . 1.0mm. 1 lb. Non-sterile.
Zirconium Oxide Beads 2.0mm
ZrOB20	Next Advance	1pack	189.6 EUR
Description: Zirconium Oxide beads . 2.0mm. 1 lb. Non-sterile.
Zirconium Oxide Beads 2.5mm
ZrOB25	Next Advance	1pack	189.6 EUR
Description: Zirconium Oxide beads . 2.5mm. 1 lb. Non-sterile.
Zirconium Silicate Beads 0.5mm
ZSB05	Next Advance	1pack	146.4 EUR
Description: Zirconium Silicate beads . 0.5mm. 1 lb. Non-sterile.
Zirconium Silicate Beads 1.0mm
ZSB10	Next Advance	1pack	146.4 EUR
Description: Zirconium Silicate beads . 1.0mm. 1 lb. Non-sterile.
Rtg Rapd Analysis Beads
27950001	Scientific Laboratory Supplies	EACH	804.84 EUR
Glass Calibration Beads 4mm
SV144	Scientific Laboratory Supplies	500G	14.71 EUR
Protect Beads Mixed Colours
SWA1147	Scientific Laboratory Supplies	PK80	103.74 EUR
Calibration Beads for MoFlo
B28479	Spherotech	each	346.8 EUR
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Magnetic Stirrer
abx725038-1Unit	Abbexa	1 Unit	518.4 EUR

Magnetic Stand
abx799001-1Unit	Abbexa	1 Unit	309.6 EUR

Magnetic Separator
B23803	Bimake	2 mL/200 uL/15 mL	291.6 EUR
Description: This Magnetic Separator is a proprietary machine, supportinga the magnetic beads series of products. A core tenent is the use of a superior magnetic, to ensure that magnetic separation step can be completed in a short time.
Magnetic Separator
MagSep1-1	ABTBeads	1/pk	132 EUR
Magnetic Stand
HY-K0200	MedChemExpress	200 μL-2 mL-15 mL	456 EUR
Magnetic Stirrer
H3760-SE	Benchmark Scientific	1 PC	121.85 EUR

Magnetic Stirrer
H4000-S-E	Benchmark Scientific	1 PC	97.22 EUR

Beads-for-Seeds (24 glass beads, each in a 1.5 ml tube)
M-CO-501	MiTeGen	24 glass beads	71 EUR

Description: Beads-for-Seeds (24 glass beads, each in a 1.5 ml tube)
Glass Beads 0.1mm RNase Free
GB01-RNA	Next Advance	1pack	115.2 EUR
Description: Glass beads. 0.1mm. 4mL. RNase free.
Glass Beads 0.5mm RNase Free
GB05-RNA	Next Advance	1pack	115.2 EUR
Description: Glass beads. 0.5mm. 4mL. RNase free.
Stainless Steel UFO Beads 3.5mm
SSUFO35	Next Advance	1pack	229.2 EUR
Description: Stainless steel UFO beads. 3.5mm. 50mL. Non-sterile.
Stainless Steel UFO Beads 5.6mm
SSUFO56	Next Advance	1pack	225.6 EUR
Description: Stainless steel UFO beads. 5.6mm. 50mL. Non-sterile.
Raf-1 RBD Agarose Beads
STA-410	Cell Biolabs	400 ?g	686.4 EUR
Description: Raf-1 RBD Agarose Beads selectively pull down the active form of Ras. Beads are colored to allow for a visual check. These are the same beads supplied with our Ras Activation Assays. 400 µg.
500GM Glass beads 2mm 500gm
GS/2	Scientific Laboratory Supplies	500G	15.28 EUR
Flexi-Block + Beads Double Width
BLO2162	Scientific Laboratory Supplies	EACH	283.86 EUR
Flexi-Block + Beads Triple Width
BLO2163	Scientific Laboratory Supplies	EACH	428.64 EUR
Protect Beads Green PP Tray
SWA1164	Scientific Laboratory Supplies	PK100	166.44 EUR
Protect Beads Red PP Tray
SWA1166	Scientific Laboratory Supplies	PK100	166.44 EUR
Protect Beads White PP Tray
SWA1168	Scientific Laboratory Supplies	PK100	166.44 EUR
Protect Beads Blue PP Tray
SWA1170	Scientific Laboratory Supplies	PK100	166.44 EUR
Protect Beads Yellow Bulk Pack
SWA1174	Scientific Laboratory Supplies	PK1000	956.46 EUR
Protect Beads Green Bulk Pack
SWA1176	Scientific Laboratory Supplies	PK1000	956.46 EUR
Protect Beads Red Bulk Pack
SWA1178	Scientific Laboratory Supplies	PK1000	956.46 EUR
Protect Beads White Bulk Pack
SWA1180	Scientific Laboratory Supplies	PK1000	956.46 EUR
Protect Beads Blue Bulk Pack
SWA1182	Scientific Laboratory Supplies	PK1000	956.46 EUR
Calibration Beads for MoFlo Astrios
B27034	Spherotech	each	346.8 EUR
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Carboxyl Magnetic Particles
CM-025-10	Spherotech	10 mL	249.6 EUR
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Carboxyl Magnetic Particles
CM-025-100H	Spherotech	100mL	1765.2 EUR
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Carboxyl Magnetic Particles
CM-025-10H	Spherotech	10mL	237.6 EUR
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Carboxyl Magnetic Particles
CM-05-10	Spherotech	10 mL	249.6 EUR
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Carboxyl Magnetic Particles
CM-05-10H	Spherotech	10mL	249.6 EUR
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Carboxyl Magnetic Particles
CM-08-10	Spherotech	10 mL	249.6 EUR
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Carboxyl Magnetic Particles
CM-10-10	Spherotech	10 mL	254.4 EUR
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Carboxyl Magnetic Particles
CM-10-100	Spherotech	100 mL	1618.8 EUR
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Carboxyl Magnetic Particles
CM-100-10	Spherotech	10 mL	408 EUR
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.