Interrelations between gene expression and DNA methylation patterns in bovine mammary gland

Quick Communication: Locus-specific interrelations between gene expression and DNA methylation patterns in bovine mammary gland contaminated by coagulase-positive and coagulase-negative staphylococci

Pathogens are in a position to alter the cell cycle program and immune response of the host by altering the transcription and epigenetics of genes liable for cell cycle management and irritation. On this regard, we evaluated interrelations between DNA methylation and expression of autophagy, apoptosis, and lipid metabolism-related genes in a pattern set of mammary gland secretory tissue sections derived from bovine mammary glands contaminated with coagulase-negative and coagulase-positive staphylococci.

We assessed relative transcript abundance and DNA bisulfite sequencing in loci of the ATG5, IGF1R, TERT, and DGAT1 genes. Lack of DNA methylation in ATG5 and DGAT1 loci is perhaps related to upkeep of ATG5 and DGAT1 expression whatever the well being standing of bovine mammary gland. Full methylation of intragenic CpG areas within the IGF1R locus was apparently not associated to the presence of its transcript within the investigated udder parenchyma samples.

Detected hypermethylation of the TERT upstream ingredient was related to a small quantity of TERT mRNA in bovine mammary gland, whatever the presence, or absence, of the pathogen. A big lower in TERT gene expression in tissue sections of mammary gland freed from micro organism and in these contaminated with coagulase-positive staphylococci was noticed in parenchyma samples contaminated with coagulase-negative staphylococci.

Two attainable explanations are the direct involvement of the TERT gene within the etiology of bovine mastitis or the rise of TERT mRNA because of activation of the MAPK signaling pathway in response to launch of exotoxins by coagulase-negative micro organism within the bovine mammary gland.

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Quick communication: Locus-specific interrelations between gene expression and DNA methylation patterns in bovine mammary gland contaminated by coagulase-positive and coagulase-negative staphylococci

Pathogens are in a position to alter the cell cycle program and immune response of the host by altering the transcription and epigenetics of genes liable for cell cycle management and irritation. On this regard, we evaluated interrelations between DNA methylation and expression of autophagy, apoptosis, and lipid metabolism-related genes in a pattern set of mammary gland secretory tissue sections derived from bovine mammary glands contaminated with coagulase-negative and coagulase-positive staphylococci.

We assessed relative transcript abundance and DNA bisulfite sequencing in loci of the ATG5, IGF1R, TERT, and DGAT1 genes. Lack of DNA methylation in ATG5 and DGAT1 loci is perhaps related to upkeep of ATG5 and DGAT1 expression whatever the well being standing of bovine mammary gland. Full methylation of intragenic CpG areas within the IGF1R locus was apparently not associated to the presence of its transcript within the investigated udder parenchyma samples. Detected hypermethylation of the TERT upstream ingredient was related to a small quantity of TERT mRNA in bovine mammary gland, whatever the presence, or absence, of the pathogen.

A big lower in TERT gene expression in tissue sections of mammary gland freed from micro organism and in these contaminated with coagulase-positive staphylococci was noticed in parenchyma samples contaminated with coagulase-negative staphylococci. Two attainable explanations are the direct involvement of the TERT gene within the etiology of bovine mastitis or the rise of TERT mRNA because of activation of the MAPK signaling pathway in response to launch of exotoxins by coagulase-negative micro organism within the bovine mammary gland.

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Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification.

Protein A Magnetic Beads

HY-K0203 MedChemExpress 1 mL 148.8 EUR

Protein G Magnetic Beads

HY-K0204 MedChemExpress 5 mL 445.2 EUR

Protein A Magnetic beads

EA-IP-015M Elabscience Biotech 1mL 105 EUR
Description: Primary Antibodies

Protein G Magnetic beads

EA-IP-016M Elabscience Biotech 1mL 105 EUR
Description: Primary Antibodies

Protein L Magnetic beads

EA-IP-017M Elabscience Biotech 1mL 105 EUR
Description: Primary Antibodies

Anti-Flag magnetic beads

B26101 Bimake 1 mL 579.6 EUR
Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification.

Anti-Flag magnetic beads

B26102 Bimake 5 mL 1742.4 EUR
Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification.

SILICA MAGNETIC BEADS 10ML

L5011010 Biochain 10 ml 73 EUR

Anti-Flag Magnetic Beads

HY-K0207 MedChemExpress 1 mL 570 EUR

BlackPearl® (Magnetic Beads)

20161006-1 Bio-WORLD - Ask for price

BlackPearl® (Magnetic Beads)

20161006-2 Bio-WORLD - Ask for price

BlackPearl® (Magnetic Beads)

20161006-3 Bio-WORLD - Ask for price

Magnetic Beads™ Streptavidin

SMB-B01 ACROBIOSYSTEMS 5mg 149.8 EUR
Description: The Streptavidin-Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin (SA). The beads can be used to capture the biotinylated proteins or other molecules, because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly.Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidin, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).The Streptavidin-Magnetic Beads is easy to capture the biotinylated proteins or other molecules in Chemiluminescence procedures, and the bounded protein have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

Concanavalin A Magnetic beads

EA-IP-014M Elabscience Biotech 1mL 159 EUR
Description: Primary Antibodies

IP/CoIP Kit (Magnetic Beads)

EA-IP-K007M Elabscience Biotech 50T 700 EUR
Description: Antibody Application Reagents

Protein A/G Magnetic Beads

6527-1 Biovision each 352.8 EUR

Protein A/G Magnetic Beads

HY-K0202 MedChemExpress 1 mL 157.2 EUR

Protein A/G Magnetic Beads

EA-IP-007M Elabscience Biotech 1mL 80 EUR
Description: Primary Antibodies

Anti-c-Myc Magnetic Beads

HY-K0206 MedChemExpress 5 mL 1368 EUR

Anti-tGFP magnetic beads, 1ml

TA150039 Origene Technologies GmbH 1 ml Ask for price

Anti-tGFP magnetic beads, 5ml

TA150040 Origene Technologies GmbH 5 ml Ask for price

9E10, Anti-Myc Magnetic beads

TA150044 Origene Technologies GmbH 1 ml Ask for price

9E10, Anti-Myc Magnetic beads

TA150045 Origene Technologies GmbH 5 ml Ask for price

Magnetic Beads PCR Cleanup Kit

MC004 Geneaid Biotech each 3 EUR

Magnetic Beads PCR Cleanup Kit

MC048 Geneaid Biotech each 25 EUR

Magnetic Beads PCR Cleanup Kit

MC096 Geneaid Biotech each 45 EUR

Anti-HA (Nanobody) Magnetic Beads

AE109 Abclonal 500μL 403 EUR

Anti-GFP (Nanobody) Magnetic Beads

AE079 Abclonal 500μL 312 EUR

Anti-Myc (Nanobody) Magnetic Beads

AE107 Abclonal 1000μL 213.2 EUR

Protein A/G/L Magnetic Beads

6547-1 Biovision each 405.6 EUR

Human CD33-coupled Magnetic Beads

MBC-K008 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human CD33 protein is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259(Accession # P20138-1(R69G)).

Human CD38-coupled Magnetic Beads

MBC-K010 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human CD38 protein is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300(Accession # P28907-1).

Human EGFR-coupled Magnetic Beads

MBE-K012 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human EGFR protein is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645(Accession # P00533-1).

Human PSMA-coupled Magnetic Beads

MBP-K007 ACROBIOSYSTEMS 2mg 3349.1 EUR
Description: Human PSMA protein is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750(Accession # Q04609-1).

Human ROR1-coupled Magnetic Beads

MBR-K009 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human ROR1 protein is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403(Accession # Q01973-1).

Human BCMA-coupled Magnetic Beads

MBS-K004 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human BCMA protein is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54(Accession # Q02223-1).

Human CD19-coupled Magnetic Beads

MBS-K005 ACROBIOSYSTEMS 2mg 4312.1 EUR
Description: Human CD19 protein is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291(Accession # P15391-1).

Human HER2-coupled Magnetic Beads

MBS-K006 ACROBIOSYSTEMS 500ug 2835.5 EUR
Description: Human HER2 protein is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652(Accession # P04626-1).

Human ACE2-coupled Magnetic Beads

MBS-K013 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human ACE2 protein is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740(Accession # Q9BYF1-1).

Human CD73-coupled Magnetic Beads

MBS-K022 ACROBIOSYSTEMS 2mg 3349.1 EUR
Description: Human CD73 Protein is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549(Accession # P21589-1).

Human CD47-coupled Magnetic Beads

MBS-K023 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human CD47 Protein is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139(Accession # Q08722).

Human CD3E-coupled Magnetic Beads

MBS-K026 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human CD3E Protein is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126(Accession # P07766-1).

Human CD22-coupled Magnetic Beads

MBS-K052 ACROBIOSYSTEMS 2mg 4312.1 EUR
Description: Human CD22 Protein is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 687(Accession # P20273-1).

Human DLL3-coupled Magnetic Beads

MBS-K053 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands.May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. DLL3 protein is expressed on the surface of tumor cells but not in normal adult tissues.

Human uPAR-coupled Magnetic Beads

MBS-K076 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Tumors are the result of uncontrolled proliferation of cells in different organs. Tumor development is a multistage process, that includes the generation of primary tumors, separation of tumors from primary sites, degradation of extracellular matrix (ECM), and distant metastasis of tumors. A variety of genes play important roles in the development of tumors, including the cell surface receptor urokinase-type plasminogen activator receptor (uPAR). uPAR is highly expressed in a variety of tumor cells, and a variety of signals regulated by uPAR play significant roles in tumor cell proliferation and metastasis, tumor-related glycolysis, the tumor microenvironment and angiogenesis. Studies have found that some specific drugs and antibodies have unique inhibitory effects on uPAR.

DDDDK-Tag Antibody (Magnetic Beads)

abx005594-100l Abbexa 100 µl 250 EUR

Amylose BlackPearl® (Magnetic Beads)

20161009-1 Bio-WORLD - Ask for price

Amylose BlackPearl® (Magnetic Beads)

20161009-2 Bio-WORLD - Ask for price

Human PCSK9-coupled Magnetic Beads

MBS-K054 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Human ASGR1-coupled Magnetic Beads

MBS-K055 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: ASGPR, a transmembrane C-type lectin, recognizes a wide variety of ligands that contain either terminal galactose (Gal) or N-acetylgalactosamine (GalNAc) residues and has been identified as a highly selective receptor on hepatocytes. The ASGR is composed of both a major (ASGR1) and minor subunit (ASGR2). ASGR1 has been shown to be efficiently targeted to the plasma membrane and to undergo constitutive endocytosis and recycling and found mainly expressed in the human liver.

Human ANGPT2-coupled Magnetic Beads

MBS-K035 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human ANGPT2 Protein is expressed from human 293 cells (HEK293). It contains AA Lys 275 - Phe 496(Accession # O15123-1).

Human VEGF165-coupled Magnetic Beads

MBS-K036 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human VEGF165 Protein is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191(Accession # P15692-4).

Ni-NTA Magnetic Agarose Beads

20-512M1 Genesee Scientific 2ml/Unit 116.39 EUR
Description: Suspension, 5% Magnetic Resin

Ni-NTA Magnetic Agarose Beads

20-512M2 Genesee Scientific 5ml/Unit 263.6 EUR
Description: Suspension, 5% Magnetic Resin

Ni-NTA Magnetic Agarose Beads

20-512M3 Genesee Scientific 10ml/Unit 477.13 EUR
Description: Suspension, 5% Magnetic Resin

Human EGFRvIII-coupled Magnetic Beads

MBS-K020 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human EGFRvIII Protein is expressed from human 293 cells (HEK293). It contains AA Leu 25- Ser 378(Accession # NP_001333870.1).

Human HGF R-coupled Magnetic Beads

MBS-K021 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human HGF R Protein is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932(Accession # P08581).

Streptavidin BlackPearl® (Magnetic Beads)

20161004-1 Bio-WORLD - Ask for price

Streptavidin BlackPearl® (Magnetic Beads)

20161004-2 Bio-WORLD - Ask for price

Human Mesothelin-coupled Magnetic Beads

MBS-K024 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human Mesothelin Protein is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580(Accession # Q13421-3(R309P)).

Human CTLA-4 coupled Magnetic Beads

MBS-K042 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human CTLA-4 Protein is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162(Accession # Q6GR94-1).

Protein A/G Magnetic Beads for IP

B23201 Bimake 1 ml 158.4 EUR
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.

Protein A/G Magnetic Beads for IP

B23202 Bimake 5 ml 558 EUR
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.

Protein A BlackPearl® (Magnetic Beads)

20161000-1 Bio-WORLD - Ask for price

Protein A BlackPearl® (Magnetic Beads)

20161000-2 Bio-WORLD - Ask for price

Protein A BlackPearl® (Magnetic Beads)

20161000-3 Bio-WORLD - Ask for price

Protein G BlackPearl® (Magnetic Beads)

20161003-1 Bio-WORLD - Ask for price

Protein G BlackPearl® (Magnetic Beads)

20161003-2 Bio-WORLD - Ask for price

Nickel (Ni2+) BlackPearl® (Magnetic Beads)

20161010-1 Bio-WORLD - Ask for price

Nickel (Ni2+) BlackPearl® (Magnetic Beads)

20161010-2 Bio-WORLD - Ask for price

Human TROP-2-coupled Magnetic Beads

MBS-K028 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human TROP-2 Protein is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 274(Accession # P09758-1).

Magnetic Beads Micro gDNA Extraction Kit

MM004 Geneaid Biotech each 3 EUR

Magnetic Beads Micro gDNA Extraction Kit

MM048 Geneaid Biotech each 63 EUR

Magnetic Beads Micro gDNA Extraction Kit

MM096 Geneaid Biotech each 113 EUR

Anti-mCherry (Nanobody) Magnetic Beads Ab

AE094 Abclonal 1000μL 213.2 EUR

Human Nectin-4-coupled Magnetic Beads

MBS-K039 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human Nectin-4 Protein is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349(Accession # Q96NY8-1).

Calmodulin (CAM) BlackPearl® (Magnetic Beads)

20161002-1 Bio-WORLD - Ask for price

Calmodulin (CAM) BlackPearl® (Magnetic Beads)

20161002-2 Bio-WORLD - Ask for price

Calmodulin (CAM) BlackPearl® (Magnetic Beads)

20161002-3 Bio-WORLD - Ask for price

Human B7-H3(4Ig)-coupled Magnetic Beads

MBS-K040 ACROBIOSYSTEMS 2mg 2707.1 EUR
Description: Human B7-H3 Protein is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461(Accession # Q5ZPR3-1).

Glutathione (GSH) BlackPearl® (Magnetic Beads)

20161007-1 Bio-WORLD - Ask for price

Glutathione (GSH) BlackPearl® (Magnetic Beads)

20161007-2 Bio-WORLD - Ask for price

Glutathione (GSH) BlackPearl® (Magnetic Beads)

20161007-3 Bio-WORLD - Ask for price

Mouse monoclonal eRFP antibody, Magnetic beads

TA183034 Origene Technologies GmbH 1 ml Ask for price

Mouse monoclonal eCFP antibody, Magnetic beads

TA183036 Origene Technologies GmbH 1 ml Ask for price

Mouse monoclonal mRFP antibody, Magnetic beads

TA183041 Origene Technologies GmbH 1 ml Ask for price

Mouse monoclonal mRFP antibody, Magnetic beads

TA183042 Origene Technologies GmbH 1 ml Ask for price

Mouse monoclonal mRFP antibody, Magnetic beads

TA183043 Origene Technologies GmbH 1 ml Ask for price

Mouse monoclonal mPlum antibody, Magnetic beads

TA183037 Origene Technologies GmbH 1 ml Ask for price

Clone OTI4C5, Anti-DDK (FLAG) Magnetic beads

TA150036 Origene Technologies GmbH 5 ml Ask for price

Clone OTI4C5, Anti-DDK (FLAG) Magnetic beads

TA150042 Origene Technologies GmbH 1 ml Ask for price

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