Interrelations between gene expression and DNA methylation patterns in bovine mammary gland

21 września, 2020 Dominika Zostaw komentarz

Quick Communication: Locus-specific interrelations between gene expression and DNA methylation patterns in bovine mammary gland contaminated by coagulase-positive and coagulase-negative staphylococci

Pathogens are in a position to alter the cell cycle program and immune response of the host by altering the transcription and epigenetics of genes liable for cell cycle management and irritation. On this regard, we evaluated interrelations between DNA methylation and expression of autophagy, apoptosis, and lipid metabolism-related genes in a pattern set of mammary gland secretory tissue sections derived from bovine mammary glands contaminated with coagulase-negative and coagulase-positive staphylococci.

We assessed relative transcript abundance and DNA bisulfite sequencing in loci of the ATG5, IGF1R, TERT, and DGAT1 genes. Lack of DNA methylation in ATG5 and DGAT1 loci is perhaps related to upkeep of ATG5 and DGAT1 expression whatever the well being standing of bovine mammary gland. Full methylation of intragenic CpG areas within the IGF1R locus was apparently not associated to the presence of its transcript within the investigated udder parenchyma samples.

Detected hypermethylation of the TERT upstream ingredient was related to a small quantity of TERT mRNA in bovine mammary gland, whatever the presence, or absence, of the pathogen. A big lower in TERT gene expression in tissue sections of mammary gland freed from micro organism and in these contaminated with coagulase-positive staphylococci was noticed in parenchyma samples contaminated with coagulase-negative staphylococci.

Two attainable explanations are the direct involvement of the TERT gene within the etiology of bovine mastitis or the rise of TERT mRNA because of activation of the MAPK signaling pathway in response to launch of exotoxins by coagulase-negative micro organism within the bovine mammary gland.

QPCR Kit DNA Universal m Mmix
MOL6612	Scientific Laboratory Supplies	EACH	1127.46 EUR
Methylamp Universal Methylated DNA Preparation Kit
P-1019	EpiGentek	40 µg	416.7 EUR
Genorise® Universal DNA Mini Kit 50
GR101076	Genorise Scientific	50	267.6 EUR
Genorise® Universal DNA Mini Kit 100
GR101076-100	Genorise Scientific	100	428.4 EUR
Genorise® Universal DNA Mini Kit 200
GR101077	Genorise Scientific	200	680.4 EUR
Genorise® Universal DNA Mini Kit 500
GR101077-500	Genorise Scientific	500	1526.4 EUR
EpiMag High Throughput DNA Isolation Universal Kit
P-1022-96	EpiGentek	96 Samples	227.7 EUR
2K DNA Marker
20-abx098045	Abbexa	309.60 EUR 226.80 EUR	2.5 ml 500 ul

5K DNA Marker
20-abx098048	Abbexa	309.60 EUR 226.80 EUR	2.5 ml 500 ul

15K DNA Marker
20-abx098049	Abbexa	326.40 EUR 226.80 EUR	2.5 ml 500 ul

1Kb DNA Marker
20-abx098050	Abbexa	309.60 EUR 226.80 EUR	2.5 ml 500 ul

100bp DNA Marker
20-abx098052	Abbexa	326.40 EUR 226.80 EUR	2.5 ml 500 ul

100bp DNA Marker
D1100-050	GenDepot	50㎍	198 EUR
100bp DNA Marker
D1100-250	GenDepot	5X50㎍	660 EUR
1Kb DNA Marker
D1101-050	GenDepot	50㎍	157.2 EUR
1Kb DNA Marker
D1101-250	GenDepot	5X50㎍	360 EUR
DL5000 DNA Marker
MD102-01	Vazyme	250 μl	128.4 EUR
DL5000 DNA Marker
MD102-02	Vazyme	500 μl	135.6 EUR
DL15000 DNA Marker
MD103-01	Vazyme	250 μl	130.8 EUR
DL15000 DNA Marker
MD103-02	Vazyme	500 μl	138 EUR
VAHTS Universal DNA Library Prep Kit for MGI
NDM607-01	Vazyme	24 rxn	879.6 EUR
VAHTS Universal DNA Library Prep Kit for MGI
NDM607-02	Vazyme	96 rxn	2563.2 EUR
1Kb Plus DNA Marker
20-abx098051	Abbexa	360.00 EUR 243.60 EUR	2.5 ml 500 ul

DL2000 Plus DNA Marker
MD101-01	Vazyme	250 μl	128.4 EUR
DL2000 Plus DNA Marker
MD101-02	Vazyme	500 μl	135.6 EUR
Linear Kinetoplast DNA Marker
TG2018-1	TopoGen	10 ug	214.8 EUR
Decatenated Kinetoplast DNA Marker
TG2020-1	TopoGen	10 ug	228 EUR
Relaxed pRYG DNA Marker
TG2025-1	TopoGen	10 ug	321.6 EUR
Linear pRYG DNA Marker
TG2028-1	TopoGen	10 ug	214.8 EUR
VAHTS Universal DNA Library Prep Kit for Illumina V3
ND607-01	Vazyme	24 rxn	529.2 EUR
VAHTS Universal DNA Library Prep Kit for Illumina V3
ND607-02	Vazyme	96 rxn	1686 EUR
VAHTS Universal Pro DNA Library Prep Kit for Illumina
ND608-01	Vazyme	24 rxn	558 EUR
VAHTS Universal Pro DNA Library Prep Kit for Illumina
ND608-02	Vazyme	96 rxn	1858.8 EUR
VAHTS Universal Plus DNA Library Prep Kit for Illumina
ND617-01	Vazyme	24 rxn	590.4 EUR
VAHTS Universal Plus DNA Library Prep Kit for Illumina
ND617-02	Vazyme	96 rxn	1926 EUR
VAHTS Universal DNA Library Prep Kit for Ion Torrent
ND701-01	Vazyme	24 rxn	879.6 EUR
VAHTS Universal DNA Library Prep Kit for Ion Torrent
ND701-02	Vazyme	96 rxn	2563.2 EUR
VAHTS Universal Pro DNA Library Prep Kit for MGI
NDM608-01	Vazyme	24 rxn	907.2 EUR
VAHTS Universal Pro DNA Library Prep Kit for MGI
NDM608-02	Vazyme	96 rxn	2740.8 EUR
VAHTS Universal Plus DNA Library Prep Kit for MGI
NDM617-01	Vazyme	24 rxn	988.8 EUR
VAHTS Universal Plus DNA Library Prep Kit for MGI
NDM617-02	Vazyme	96 rxn	2889.6 EUR
1 Kb Ladder DNA Marker
FYD001-500UL	Yeastern Biotech	20 µg/ 500 µl	Ask for price
100 bp Ladder DNA Marker
FYD002-500UL	Yeastern Biotech	30 µg/ 500 µl	Ask for price
50 bp Ladder DNA Marker
FYD004-500UL	Yeastern Biotech	32.5 µg/ 500 µl	Ask for price
Universal platform
INC1112	Scientific Laboratory Supplies	EACH	1265.4 EUR
Universal Attachment
MIX1320	Scientific Laboratory Supplies	EACH	272.46 EUR
500-10000bp DNA Marker, Original Form
M101O-1	Bio Basic	100loading, 100prep	110.11 EUR

500-10000bp DNA Marker, Original Form
M101O-2	Bio Basic	5x100loading, 500prep	263.58 EUR

100-1500bp DNA Marker, Original Form
M102O-1	Bio Basic	100loading, 100prep	108.02 EUR

100-1500bp DNA Marker, Original Form
M102O-2	Bio Basic	5x100loading, 500prep	258.36 EUR

100-1500bp DNA Marker, Original Form
M107O-1	Bio Basic	100loading, 100prep	110.11 EUR

100-1500bp DNA Marker, Original Form
M107O-2	Bio Basic	5x100loading, 500prep	263.58 EUR

50-500bp Low Range DNA Marker
GM305	Bio Basic	200loading, 200prep	137.78 EUR

Image Green? 100 bp DNA Marker
M1155-500	Biovision	each	352.8 EUR
Image Green? 1 kb DNA Marker
M1156-500	Biovision	each	352.8 EUR
VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)
ND607-03	Vazyme	24 rxn	514.8 EUR
VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)
ND607-04	Vazyme	96 rxn	1624.8 EUR
2K Plus DNA Marker (8 linear double-stranded DNA bands)
20-abx098046	Abbexa	309.60 EUR 226.80 EUR	2.5 ml 500 ul

2K Plus DNA Marker (9 linear double-stranded DNA bands)
20-abx098047	Abbexa	309.60 EUR 226.80 EUR	2.5 ml 500 ul

100bp Plus DNA Marker (12 linear double-stranded DNA bands)
20-abx098053	Abbexa	326.40 EUR 226.80 EUR	2.5 ml 500 ul

100bp Plus DNA Marker (14 linear double-stranded DNA bands)
20-abx098054	Abbexa	360.00 EUR 243.60 EUR	2.5 ml 500 ul

Universal Detachment Solution
AD002	Neuromics	100 ml	177.6 EUR
Universal Neutralization Buffer
NB001	Neuromics	100 ml	162 EUR
Universal Spring Platform
H1000-P-SP	Benchmark Scientific	1 PC	388.07 EUR

Universal qPCR Mixture
QPCR1000-UNIV	Bio Basic	4X1.25ml	331.44 EUR

UNIVERSAL BEER AGAR
U21-100-10kg	Alphabiosciences	10 kg	1828.8 EUR
UNIVERSAL BEER AGAR
U21-100-2kg	Alphabiosciences	2kg	444 EUR
UNIVERSAL BEER AGAR
U21-100-500g	Alphabiosciences	500 g	164.4 EUR
UNIVERSAL PREENRICHMENT BROTH
U21-101-10kg	Alphabiosciences	10 kg	898.8 EUR
UNIVERSAL PREENRICHMENT BROTH
U21-101-2Kg	Alphabiosciences	2 Kg	242.4 EUR
UNIVERSAL PREENRICHMENT BROTH
U21-101-500g	Alphabiosciences	500 g	109.2 EUR
Mains adaptor (universal)
ARG1248	Scientific Laboratory Supplies	EACH	118.56 EUR
Grant Universal Tray
BAT4622	Scientific Laboratory Supplies	EACH	275.88 EUR
Universal Spring Tray
BAT7177	Scientific Laboratory Supplies	EACH	259.92 EUR
Universal Stratus Adapter
MIC6036	Scientific Laboratory Supplies	EACH	445.74 EUR
Loopster Universal Disc
MIX1080	Scientific Laboratory Supplies	EACH	171 EUR
Universal (Aluminum) Platform
MIX1322	Scientific Laboratory Supplies	EACH	132.24 EUR
Universal Spring Platform
MIX7184	Scientific Laboratory Supplies	EACH	738.72 EUR
IKA Universal Attachment
SHA2016	Scientific Laboratory Supplies	EACH	947.34 EUR
18mmMag Universal ScrewCap
CHR3460	Scientific Laboratory Supplies	PK1000	950.76 EUR
Universal attachment IKA
SHA2210	Scientific Laboratory Supplies	EACH	744.42 EUR
NBS Universal Platform
SHA4050	Scientific Laboratory Supplies	EACH	1288.2 EUR
NBS Universal Platform
SHA4070	Scientific Laboratory Supplies	EACH	934.8 EUR
NBS Universal Platform
SHA4232	Scientific Laboratory Supplies	EACH	513 EUR
NBS Universal Platform
SHA4256	Scientific Laboratory Supplies	EACH	628.14 EUR
NBS Universal Platform
SHA4272	Scientific Laboratory Supplies	EACH	996.36 EUR
NBS Universal Platform
SHA4290	Scientific Laboratory Supplies	EACH	1340.64 EUR
Universal AC adapter
PIP3214	Scientific Laboratory Supplies	EACH	62.93 EUR
Universal Multichannel Stand
F161417	Scientific Laboratory Supplies	EACH	28.5 EUR
Universal Oven UN30
OVE8314	Scientific Laboratory Supplies	EACH	1381.68 EUR
Universal Oven UN55
OVE8316	Scientific Laboratory Supplies	EACH	1648.44 EUR
Universal Oven UN75
OVE8318	Scientific Laboratory Supplies	EACH	1714.56 EUR
Universal Oven UN110
OVE8320	Scientific Laboratory Supplies	EACH	2194.5 EUR
Universal Oven UN160
OVE8322	Scientific Laboratory Supplies	EACH	2648.22 EUR
Universal Oven UN260
OVE8324	Scientific Laboratory Supplies	EACH	3505.5 EUR
Universal Oven UN450
OVE8326	Scientific Laboratory Supplies	EACH	4639.8 EUR
Universal Oven UN750
OVE8328	Scientific Laboratory Supplies	EACH	6305.34 EUR
Universal Oven UF30
OVE8332	Scientific Laboratory Supplies	EACH	1614.24 EUR
Universal Oven UF55
OVE8334	Scientific Laboratory Supplies	EACH	1948.26 EUR
Universal Oven UF75
OVE8336	Scientific Laboratory Supplies	EACH	2056.56 EUR
Universal Oven UF110
OVE8338	Scientific Laboratory Supplies	EACH	2401.98 EUR
Universal Oven UF160
OVE8340	Scientific Laboratory Supplies	EACH	3161.22 EUR
Universal Oven UF260
OVE8342	Scientific Laboratory Supplies	EACH	4031.04 EUR
Universal Oven UF450
OVE8344	Scientific Laboratory Supplies	EACH	5278.2 EUR
Universal Oven UF750
OVE8346	Scientific Laboratory Supplies	EACH	7125 EUR
Universal Oven UF1060
OVE8348	Scientific Laboratory Supplies	EACH	12519.48 EUR
Universal Oven UN30plus
OVE8350	Scientific Laboratory Supplies	EACH	2020.08 EUR
Universal Oven UN55plus
OVE8352	Scientific Laboratory Supplies	EACH	2204.76 EUR
Universal Oven UN75plus
OVE8354	Scientific Laboratory Supplies	EACH	2453.28 EUR
Universal Oven UN110plus
OVE8356	Scientific Laboratory Supplies	EACH	2812.38 EUR
Universal Oven UN260plus
OVE8358	Scientific Laboratory Supplies	EACH	4189.5 EUR
Universal Oven UN450plus
OVE8360	Scientific Laboratory Supplies	EACH	5400.18 EUR
Universal Oven UN750plus
OVE8362	Scientific Laboratory Supplies	EACH	7083.96 EUR
Universal Oven UF30plus
OVE8366	Scientific Laboratory Supplies	EACH	2131.8 EUR
Universal Oven UF55plus
OVE8368	Scientific Laboratory Supplies	EACH	2530.8 EUR
Universal Oven UF75plus
OVE8370	Scientific Laboratory Supplies	EACH	2789.58 EUR
Universal Oven UF110plus
OVE8372	Scientific Laboratory Supplies	EACH	3218.22 EUR
Universal Oven UF160plus
OVE8374	Scientific Laboratory Supplies	EACH	4009.38 EUR
Universal Oven UF260plus
OVE8376	Scientific Laboratory Supplies	EACH	4753.8 EUR
Universal Oven UF450plus
OVE8378	Scientific Laboratory Supplies	EACH	6065.94 EUR
Universal Oven UF750plus
OVE8380	Scientific Laboratory Supplies	EACH	8708.46 EUR
Universal Oven UF1060plus
OVE8382	Scientific Laboratory Supplies	EACH	13331.16 EUR
Universal Spring Platform
H1010-P-SP	Benchmark Scientific	1 each	677.5 EUR
Universal Indicator Solution
UN10025	Scientific Laboratory Supplies	2.5L	165.3 EUR
Universal Indicator Solution
UN1005	Scientific Laboratory Supplies	50ML	17.1 EUR
Universal Indicator Solution
UN101	Scientific Laboratory Supplies	100ML	18.24 EUR
Universal Indicator Solution
UN1025	Scientific Laboratory Supplies	25L	650.94 EUR
Universal Indicator Solution
UN105	Scientific Laboratory Supplies	500ML	90.06 EUR
Universal Indicator Solution
UNB1010	Scientific Laboratory Supplies	10L	627 EUR
Universal V1-Puck
M-CP-111-021	MiTeGen	1 PUCK	350 EUR

Description: Universal V1-Puck
Tumour Marker MUC1 (S1.1) , DNA Aptamer, Biotinylated
AD-152-B	Alpha Diagnostics	Custom	Ask for price
Tumour Marker MUC1 (S1.1) , DNA Aptamer, unlabeled
AD-152-U	Alpha Diagnostics	Custom	Ask for price
500-10000bp DNA Marker, Ready-to-use
M101R-1	Bio Basic	100loading, 100prep	110.11 EUR

500-10000bp DNA Marker, Ready-to-use
M101R-2	Bio Basic	5x100loading, 500prep	263.58 EUR

100-1500bp DNA Marker, Ready-to-use
M102R-1	Bio Basic	100loading, 100prep	108.02 EUR

100-1500bp DNA Marker, Ready-to-use
M102R-2	Bio Basic	5x100loading, 500prep	258.36 EUR

Lambda DNA/HindIII Marker, Ready-to-use
M104R-1	Bio Basic	100loading, 100prep	78.79 EUR

Lambda DNA/HindIII Marker, Ready-to-use
M104R-2	Bio Basic	5x100loading, 500prep	149.78 EUR

100-1500bp DNA Marker, Ready-to-use
M107R-1	Bio Basic	100loading, 100prep	110.11 EUR

100-1500bp DNA Marker, Ready-to-use
M107R-2	Bio Basic	5x100loading, 500prep	263.58 EUR

50-1500bp DNA Marker, Ready-to-use
M109-A	Bio Basic	100loading, 100prep	110.11 EUR

50-1500bp DNA Marker, Ready-to-use
M109-B	Bio Basic	5x100loading, 500prep	263.58 EUR

100-1200bp DNA Marker, Ready-to-use
GM333	Bio Basic	50loading, 50prep	80.88 EUR

100-1200bp DNA Marker, Ready-to-use
GM334	Bio Basic	5x50loading, 250prep	153.96 EUR

100-2000bp DNA Marker, Ready-to-use
GM335	Bio Basic	50loading, 50prep	80.88 EUR

100-2000bp DNA Marker, Ready-to-use
GM336	Bio Basic	5x50loading, 250prep	153.96 EUR

200-1500bp DNA Marker, Ready-to-use
GM337	Bio Basic	50loading, 50prep	80.88 EUR

200-1500bp DNA Marker, Ready-to-use
GM338	Bio Basic	5x50loading, 250prep	153.96 EUR

200-2000bp DNA Marker, Ready-to-use
GM339	Bio Basic	50loading, 50prep	80.88 EUR

200-2000bp DNA Marker, Ready-to-use
GM340	Bio Basic	5x50loading, 250prep	153.96 EUR

300-2500bp DNA Marker, Ready-to-use
GM341	Bio Basic	50loading, 50prep	80.88 EUR

300-2500bp DNA Marker, Ready-to-use
GM342	Bio Basic	5x50loading, 250prep	153.96 EUR

100-1000bp DNA Marker, Ready-to-use
GM343	Bio Basic	50loading, 50prep	96.54 EUR

100-1000bp DNA Marker, Ready-to-use
GM345	Bio Basic	50loading, 50prep	101.76 EUR

100-1500bp DNA Marker, Ready-to-use
SGM01	Bio Basic	50loading, 50prep	80.88 EUR

100-1500bp DNA Marker, Ready-to-use
SGM02	Bio Basic	5x50loading, 250prep	153.96 EUR

PCR plate-foil universal
A28216	GeneOn	1x100 pcs	112.8 EUR
PCR plate-foil universal
A28216L	GeneOn	5x100 pcs	372 EUR
Universal Casein Diluent/Blocker
85R-108	Fitzgerald	250 ml	276 EUR
Description: Ready-to-use biotin free reagent applicable for blocking ELISA plates
Universal Protein Precipitating Reagent
BSP012	Bio Basic	100preps	97.58 EUR

5min Universal Ligation Mix
C311-01	Vazyme	50 rxn	256.8 EUR
5min Universal Ligation Mix
C311-02	Vazyme	100 rxn	339.6 EUR
Universal Full Growth Medium
TM001	Neuromics	500 ml	403.2 EUR
Universal Ohaus Plug GB
46001779	Scientific Laboratory Supplies	EACH	13.91 EUR
Universal tray for LSB12
BAT1062	Scientific Laboratory Supplies	EACH	263.34 EUR
Universal tray for LSB18
BAT1064	Scientific Laboratory Supplies	EACH	275.88 EUR
2 Universal Clip Plates
MIX5190	Scientific Laboratory Supplies	EACH	90.06 EUR
Azo Universal spray 500ml
CLE1996	Scientific Laboratory Supplies	EACH	5.18 EUR
AED Universal Wall Bracket
SAF1128	Scientific Laboratory Supplies	EACH	84.93 EUR
18mm Mag Universal ScrewCap
CHR3466	Scientific Laboratory Supplies	PK1000	898.32 EUR
18mm Mag Universal ScrewCap
CHR3468	Scientific Laboratory Supplies	PK1000	748.98 EUR
Universal Active Charcoal Filter
FUM1146	Scientific Laboratory Supplies	EACH	465.12 EUR
Universal Attachment for SHA20702
SHA2074	Scientific Laboratory Supplies	EACH	2370.06 EUR
IKA AS4000.1 Universal Attachment
SHA2612	Scientific Laboratory Supplies	EACH	1189.02 EUR
Universal Platform Innova S44i
SHA3072	Scientific Laboratory Supplies	EACH	1279.08 EUR
Grant Universal platform 270x195mm
SHA7932	Scientific Laboratory Supplies	EACH	159.6 EUR
Universal Pipette Holder Assorted
PIP0164	Scientific Laboratory Supplies	EACH	44.57 EUR
100bp DNA Marker Plus-50, Ready-to-use
D1103-050	GenDepot	500 ul	181.2 EUR
100bp DNA Marker Plus-50, Ready-to-use
D1103-100	GenDepot	2x500 ul	230.4 EUR
100bp PCR Ranger DNA Marker, 100bp-To-3Kb
D1108-100	GenDepot	1ml	264 EUR
100bp PCR Ranger DNA Marker, 100bp-To-3Kb
D1108-200	GenDepot	2x1ml	370.8 EUR
1Kb PCR Ranger DNA Marker, 750bp-To-20Kb
D1109-100	GenDepot	1ml	225.6 EUR
Tumour Marker MUC1 (S1.1) , DNA Aptamer, FITC labeled
AD-152-F	Alpha Diagnostics	Custom	Ask for price
200-2000bp DNA Marker Plus, Ready-to-use
GM401	Bio Basic	50loading, 50prep	72.53 EUR

100-5000bp DNA Marker Plus, Ready-to-use
SGM03	Bio Basic	50loading, 50prep	80.88 EUR

1Kb PCR Ranger DNA Marker, 750bp-To-20Kb
D1109-400	GenDepot	4x1ml	465.6 EUR
Universal IHC Diluent/Blocker/Stabilizer
85R-1021	Fitzgerald	1 liter	830.4 EUR
Description: Universal Diluent/Blocker/Stabilizer for use in IHC
HotTaq Probe qPCR Universal Mix
BT11004	Bioatlas	250rxn	147.6 EUR
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
HotTaq EvaGreen qPCR Universal Mix
BT11104	Bioatlas	250rxn	165.6 EUR
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
UNIVERSAL LINEAR 4 PLACE STAND
AP-USD4	CORNING	1/pk	84 EUR
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller
VAHTS Universal Plus Fragmentation Module
N209-01	Vazyme	24 rxn	451.2 EUR
VAHTS Universal Plus Fragmentation Module
N209-02	Vazyme	96 rxn	1226.4 EUR

Quick communication: Locus-specific interrelations between gene expression and DNA methylation patterns in bovine mammary gland contaminated by coagulase-positive and coagulase-negative staphylococci

We assessed relative transcript abundance and DNA bisulfite sequencing in loci of the ATG5, IGF1R, TERT, and DGAT1 genes. Lack of DNA methylation in ATG5 and DGAT1 loci is perhaps related to upkeep of ATG5 and DGAT1 expression whatever the well being standing of bovine mammary gland. Full methylation of intragenic CpG areas within the IGF1R locus was apparently not associated to the presence of its transcript within the investigated udder parenchyma samples. Detected hypermethylation of the TERT upstream ingredient was related to a small quantity of TERT mRNA in bovine mammary gland, whatever the presence, or absence, of the pathogen.

A big lower in TERT gene expression in tissue sections of mammary gland freed from micro organism and in these contaminated with coagulase-positive staphylococci was noticed in parenchyma samples contaminated with coagulase-negative staphylococci. Two attainable explanations are the direct involvement of the TERT gene within the etiology of bovine mastitis or the rise of TERT mRNA because of activation of the MAPK signaling pathway in response to launch of exotoxins by coagulase-negative micro organism within the bovine mammary gland.

Anti-HA magnetic beads
B26201	Bimake	1 mL	579.6 EUR
Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification.
Anti-HA magnetic beads
B26202	Bimake	5 mL	1742.4 EUR
Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification.
Anti-Myc magnetic beads
B26301	Bimake	1 mL	579.6 EUR
Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification.
Anti-Myc magnetic beads
B26302	Bimake	5 mL	1742.4 EUR
Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification.
Anti-HA Magnetic Beads
HY-K0201	MedChemExpress	1 mL	334.8 EUR
Protein A Magnetic Beads
HY-K0203	MedChemExpress	1 mL	148.8 EUR
Protein G Magnetic Beads
HY-K0204	MedChemExpress	5 mL	445.2 EUR
Anti-Flag Magnetic Beads
HY-K0207	MedChemExpress	1 mL	570 EUR
Protein A Magnetic Beads
6507-1	Biovision	each	314.4 EUR
Protein G Magnetic Beads
6517-1	Biovision	each	314.4 EUR
Protein L Magnetic Beads
6537-1	Biovision	each	352.8 EUR
Protein A/G Magnetic Beads
HY-K0202	MedChemExpress	1 mL	157.2 EUR
Anti-c-Myc Magnetic Beads
HY-K0206	MedChemExpress	5 mL	1368 EUR
Protein A/G Magnetic Beads
6527-1	Biovision	each	352.8 EUR
Size Selection DNA Beads
20-abx298001	Abbexa	260.40 EUR 477.60 EUR 1663.20 EUR	1 ml 5 ml 60 ml

VAHTS DNA Clean Beads
N411-01	Vazyme	5 ml	259.2 EUR
VAHTS DNA Clean Beads
N411-02	Vazyme	60 ml	634.8 EUR
VAHTS DNA Clean Beads
N411-03	Vazyme	450 ml	3055.2 EUR
Protein A/G/L Magnetic Beads
6547-1	Biovision	each	405.6 EUR
Protein A/G Magnetic Beads for IP
B23201	Bimake	1 ml	158.4 EUR
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.
Protein A/G Magnetic Beads for IP
B23202	Bimake	5 ml	558 EUR
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.
100 BP DNA LADDER, 500UL PER KIT
M-DNA-100BP	CORNING	1/pk	87.6 EUR
Description: Bioscience Mol Bio; DNA Ladder
1 KB DNA LADDER, 500UL PER KIT
M-DNA-1KB	CORNING	1/pk	84 EUR
Description: Bioscience Mol Bio; DNA Ladder
Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb
AE037	Abclonal	50 ul	211.2 EUR
FAB & Beads(Ballotini Beads)
MED1042	Scientific Laboratory Supplies	PK50	91.2 EUR
Circulating DNA Kit (with Magnetic Stand)
abx098869-50rxns	Abbexa	50 rxns	510 EUR

Circulating DNA Kit (with Magnetic Stand)
abx298026-50rxns	Abbexa	50 rxns	510 EUR

RNA/DNA Purification Kit (Magnetic Bead)
DA0623	Daan Gene	32 test/kit	Ask for price
Blood Genomic DNA Kit (with Magnetic Stand)
abx098867-50rxns	Abbexa	50 rxns	360 EUR

Viral DNA / RNA Kit (with Magnetic Stand)
abx098871-50rxns	Abbexa	50 rxns	477.6 EUR

Viral DNA/RNA Kit (with Magnetic Stand)
abx298029-50rxns	Abbexa	50 rxns	477.6 EUR

Latex Beads
abx291003-1L	Abbexa	1 L	2798.4 EUR

RNA Beads
20-abx298002	Abbexa	260.40 EUR 477.60 EUR 1663.20 EUR	1 ml 5 ml 60 ml

Bulk Beads
D1131-01	Benchmark Scientific	1 PC	285.76 EUR

Bulk Beads
D1131-05	Benchmark Scientific	1 PC	285.76 EUR

Bulk Beads
D1131-10	Benchmark Scientific	1 PC	492.47 EUR

Bulk Beads
D1132-01TP	Benchmark Scientific	1 PC	492.47 EUR

Bulk Beads
D1132-05TP	Benchmark Scientific	1 PC	492.47 EUR

Bulk Beads
D1132-10TP	Benchmark Scientific	1 PC	492.47 EUR

Bulk Beads
D1132-15TP	Benchmark Scientific	1 PC	492.47 EUR

Bulk Beads
D1132-30TP	Benchmark Scientific	1 PC	492.47 EUR

Bulk Beads
D1132-60	Benchmark Scientific	1 PC	289.93 EUR

Bulk Beads
D1133-28	Benchmark Scientific	1 PC	112.45 EUR

Exorose Beads
EXOB-100	ABTBeads	100 ml	127.2 EUR
Exorose Beads
EXOB-25	ABTBeads	25 ml	69.6 EUR
Exorose Beads
EXOB-250	ABTBeads	250 ml	225.6 EUR
Saline & Beads
MED1064	Scientific Laboratory Supplies	PK50	91.2 EUR
Refill Glass Beads
B1201-BEAD	Benchmark Scientific	1 PC	141.34 EUR

V5 agarose beads
AE029-100ul	Abclonal	100 ul	Ask for price
V5 agarose beads
AE029-200ul	Abclonal	200 ul	273.6 EUR
V5 agarose beads
AE029-20ul	Abclonal	20 ul	Ask for price
V5 agarose beads
AE029-50ul	Abclonal	50 ul	Ask for price
Glass Beads 0.1mm
GB01	Next Advance	1pack	129.6 EUR
Description: Glass beads. 0.1mm. 1 lb. Non-sterile.
Glass Beads 0.5mm
GB05	Next Advance	1pack	123.6 EUR
Description: Glass beads. 0.5mm. 1 lb. Non-sterile.
Glass Beads 1.0mm
GB10	Next Advance	1pack	123.6 EUR
Description: Glass beads. 1.0mm. 1 lb. Non-sterile.
Sodium hydroxide, Beads
SB6789	Bio Basic	500g	73.21 EUR

RanBP1 Agarose Beads
STA-421	Cell Biolabs	400 µg	686.4 EUR
Description: RanBP1 Agarose Beads selectively pull down the active form of Ran. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.
Protect beads green
SWA1151	Scientific Laboratory Supplies	PK80	103.74 EUR
Protect Beads White
SWA1155	Scientific Laboratory Supplies	PK80	103.74 EUR
Protect Beads Blue
SWA1158	Scientific Laboratory Supplies	PK80	103.74 EUR
Thrombin Sepharose Beads
7925-1	Biovision	each	261.6 EUR
Thrombin Sepharose Beads
7925-25	Biovision	each	2114.4 EUR
Thrombin Sepharose Beads
7925-5	Biovision	each	836.4 EUR
Plasmin Sepharose Beads
7926-1	Biovision	each	261.6 EUR
Plasmin Sepharose Beads
7926-25	Biovision	each	2114.4 EUR
Plasmin Sepharose Beads
7926-5	Biovision	each	836.4 EUR
Urokinase Sepharose Beads
7927-1	Biovision	each	261.6 EUR
Urokinase Sepharose Beads
7927-25	Biovision	each	2114.4 EUR
Urokinase Sepharose Beads
7927-5	Biovision	each	836.4 EUR
Immobilized Catalase Beads
7931-1	Biovision	each	183.6 EUR
Immobilized Catalase Beads
7931-10	Biovision	each	705.6 EUR
Calmodulin-Sepharose Beads
7934-1	Biovision	each	183.6 EUR
Calmodulin-Sepharose Beads
7934-10	Biovision	each	574.8 EUR
Calmodulin-Sepharose Beads
7934-5	Biovision	each	405.6 EUR
Streptavidin-Sepharose Beads
6565-10	Biovision	each	836.4 EUR
Streptavidin-Sepharose Beads
6565-100	Biovision	each	Ask for price
Streptavidin-Sepharose Beads
6565-2	Biovision	each	274.8 EUR
Streptavidin-Sepharose Beads
6565-5	Biovision	each	483.6 EUR
EZEnrich? Polyubiquitin Beads
6568-300	Biovision	each	444 EUR
VAHTS mRNA Capture Beads
N401-01	Vazyme	24 rxn	171.6 EUR
VAHTS mRNA Capture Beads
N401-02	Vazyme	96 rxn	306 EUR
VAHTS RNA Clean Beads
N412-01	Vazyme	5 ml	286.8 EUR
VAHTS RNA Clean Beads
N412-02	Vazyme	40 ml	495.6 EUR
VAHTS RNA Clean Beads
N412-03	Vazyme	450 ml	4860 EUR
Stainless Steel Beads 0.2mm
SSB02	Next Advance	1pack	229.2 EUR
Description: Stainless steel beads. 0.2mm. 1 lb. Non-sterile.
Stainless Steel Beads 0.5mm
SSB05	Next Advance	1pack	229.2 EUR
Description: Stainless steel beads. 0.5mm. 1 lb. Non-sterile.
Stainless Steel Beads 1.6mm
SSB16	Next Advance	1pack	304.8 EUR
Description: Stainless steel beads. 1.6mm. 1 lb. Non-sterile.
Stainless Steel Beads 2.3mm
SSB23	Next Advance	1pack	260.4 EUR
Description: Stainless steel beads. 2.3mm. 1 lb. Non-sterile.
Stainless Steel Beads 3.2mm
SSB32	Next Advance	1pack	220.8 EUR
Description: Stainless steel beads. 3.2mm. 1 lb. Non-sterile.
Stainless Steel Beads 4.8mm
SSB48	Next Advance	1pack	220.8 EUR
Description: Stainless steel beads. 4.8mm. 1 lb. Non-sterile.
Stainless Steel Beads 6.0mm
SSB60	Next Advance	1pack	223.2 EUR
Description: Stainless steel beads. 6.0mm. 1 lb. Non-sterile.
PAK1 PBD Agarose Beads
STA-411	Cell Biolabs	400 ?g	686.4 EUR
Description: PAK1 PBD Agarose Beads selectively pull down the active form of Rac and Cdc42. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.
Rhotekin RBD Agarose Beads
STA-412	Cell Biolabs	400 ?g	686.4 EUR
Description: Rhotekin RBD Agarose Beads selectively pull down the active form of Rho. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.
RalGDS RBD Agarose Beads
STA-418	Cell Biolabs	400 µg	686.4 EUR
Description: RalGDS RBD Agarose Beads selectively pull down the active form of Rap. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.
GGA3 PBD Agarose Beads
STA-419	Cell Biolabs	400 µg	686.4 EUR
Description: GGA3 PBD Agarose Beads selectively pull down the active form of Arf. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.
RalBP1 PBD Agarose Beads
STA-420	Cell Biolabs	400 µg	686.4 EUR
Description: RalBP1 RBD Agarose Beads selectively pull down the active form of Ral. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.
Zirconium Oxide Beads 0.15mm
ZrOB015	Next Advance	1pack	189.6 EUR
Description: Fused Zirconium Oxide beads . 0.15mm. 1 lb. Non-sterile.
Zirconium Oxide Beads 0.5mm
ZrOB05	Next Advance	1pack	189.6 EUR
Description: Zirconium Oxide beads . 0.5mm. 1 lb. Non-sterile.
Zirconium Oxide Beads 1.0mm
ZrOB10	Next Advance	1pack	189.6 EUR
Description: Zirconium Oxide beads . 1.0mm. 1 lb. Non-sterile.
Zirconium Oxide Beads 2.0mm
ZrOB20	Next Advance	1pack	189.6 EUR
Description: Zirconium Oxide beads . 2.0mm. 1 lb. Non-sterile.
Zirconium Oxide Beads 2.5mm
ZrOB25	Next Advance	1pack	189.6 EUR
Description: Zirconium Oxide beads . 2.5mm. 1 lb. Non-sterile.
Zirconium Silicate Beads 0.5mm
ZSB05	Next Advance	1pack	146.4 EUR
Description: Zirconium Silicate beads . 0.5mm. 1 lb. Non-sterile.
Zirconium Silicate Beads 1.0mm
ZSB10	Next Advance	1pack	146.4 EUR
Description: Zirconium Silicate beads . 1.0mm. 1 lb. Non-sterile.
Rtg Rapd Analysis Beads
27950001	Scientific Laboratory Supplies	EACH	804.84 EUR
Glass Calibration Beads 4mm
SV144	Scientific Laboratory Supplies	500G	14.71 EUR
Protect Beads Mixed Colours
SWA1147	Scientific Laboratory Supplies	PK80	103.74 EUR
Calibration Beads for MoFlo
B28479	Spherotech	each	346.8 EUR
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Magnetic Stirrer
abx725038-1Unit	Abbexa	1 Unit	518.4 EUR

Magnetic Stand
abx799001-1Unit	Abbexa	1 Unit	309.6 EUR

Magnetic Separator
B23803	Bimake	2 mL/200 uL/15 mL	291.6 EUR
Description: This Magnetic Separator is a proprietary machine, supportinga the magnetic beads series of products. A core tenent is the use of a superior magnetic, to ensure that magnetic separation step can be completed in a short time.
Magnetic Separator
MagSep1-1	ABTBeads	1/pk	132 EUR
Magnetic Stirrer
H3760-SE	Benchmark Scientific	1 PC	121.85 EUR

Magnetic Stirrer
H4000-S-E	Benchmark Scientific	1 PC	97.22 EUR

Magnetic Stand
HY-K0200	MedChemExpress	200 μL-2 mL-15 mL	456 EUR
Beads-for-Seeds (24 glass beads, each in a 1.5 ml tube)
M-CO-501	MiTeGen	24 glass beads	71 EUR

Description: Beads-for-Seeds (24 glass beads, each in a 1.5 ml tube)
Glass Beads 0.1mm RNase Free
GB01-RNA	Next Advance	1pack	115.2 EUR
Description: Glass beads. 0.1mm. 4mL. RNase free.
Glass Beads 0.5mm RNase Free
GB05-RNA	Next Advance	1pack	115.2 EUR
Description: Glass beads. 0.5mm. 4mL. RNase free.
Stainless Steel UFO Beads 3.5mm
SSUFO35	Next Advance	1pack	229.2 EUR
Description: Stainless steel UFO beads. 3.5mm. 50mL. Non-sterile.
Stainless Steel UFO Beads 5.6mm
SSUFO56	Next Advance	1pack	225.6 EUR
Description: Stainless steel UFO beads. 5.6mm. 50mL. Non-sterile.
Raf-1 RBD Agarose Beads
STA-410	Cell Biolabs	400 ?g	686.4 EUR
Description: Raf-1 RBD Agarose Beads selectively pull down the active form of Ras. Beads are colored to allow for a visual check. These are the same beads supplied with our Ras Activation Assays. 400 µg.
Flexi-Block + Beads Double Width
BLO2162	Scientific Laboratory Supplies	EACH	283.86 EUR
Flexi-Block + Beads Triple Width
BLO2163	Scientific Laboratory Supplies	EACH	428.64 EUR
500GM Glass beads 2mm 500gm
GS/2	Scientific Laboratory Supplies	500G	15.28 EUR
Protect Beads Green PP Tray
SWA1164	Scientific Laboratory Supplies	PK100	166.44 EUR
Protect Beads Red PP Tray
SWA1166	Scientific Laboratory Supplies	PK100	166.44 EUR
Protect Beads White PP Tray
SWA1168	Scientific Laboratory Supplies	PK100	166.44 EUR
Protect Beads Blue PP Tray
SWA1170	Scientific Laboratory Supplies	PK100	166.44 EUR
Protect Beads Yellow Bulk Pack
SWA1174	Scientific Laboratory Supplies	PK1000	956.46 EUR
Protect Beads Green Bulk Pack
SWA1176	Scientific Laboratory Supplies	PK1000	956.46 EUR
Protect Beads Red Bulk Pack
SWA1178	Scientific Laboratory Supplies	PK1000	956.46 EUR
Protect Beads White Bulk Pack
SWA1180	Scientific Laboratory Supplies	PK1000	956.46 EUR
Protect Beads Blue Bulk Pack
SWA1182	Scientific Laboratory Supplies	PK1000	956.46 EUR
Calibration Beads for MoFlo Astrios
B27034	Spherotech	each	346.8 EUR
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Amino Magnetic Particles
AM-10-10	Spherotech	10 mL	254.4 EUR
Description: Amino Superparamagnetic Particles are recomended for variety of applications such as cell separation, affinity purification, DNA probe assays, magnetic particle EIA, etc. The key features are does not contain any free iron oxide, thus reducing undesirable interference, you may obtain iable and functionally active cells using using magnetic labeling after coupling with cell surface molecules.
Amino Magnetic Particles
AM-10-100	Spherotech	100 mL	1618.8 EUR
Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Amino Magnetic Particles
AM-15-10	Spherotech	10 mL	254.4 EUR
Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Amino Magnetic Particles
AM-15-100	Spherotech	100 mL	1618.8 EUR
Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Amino Magnetic Particles
AM-20-10	Spherotech	10 mL	261.6 EUR
Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Amino Magnetic Particles
AM-20-100	Spherotech	100 mL	1668 EUR
Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Amino Magnetic Particles
AM-30-10	Spherotech	10 mL	261.6 EUR
Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Amino Magnetic Particles
AM-30-100	Spherotech	100 mL	1668 EUR
Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.
Amino Magnetic Particles
AM-40-10	Spherotech	10 mL	261.6 EUR
Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.